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A second person has died from injuries sustained in a two-vehicle collision near Andoversford yesterday, Monday 17 January.
Investigating officers are now appealing for dashcam footage and witnesses of the fatal collision which happened late morning.
Police were called to the A436 near Pegglesworth shortly after 11.15am with a report of a collision which involved a white heavy goods vehicle (HGV) and a black Mazda.
The Mazda had been travelling toward Seven Springs from the direction of Andoversford and the HGV was travelling in the opposite direction.
The driver of the car, a 31-year-old man, was pronounced deceased at the scene.
A 24-year-old woman died from her injuries in hospital last night, Monday 17 January.
Three other passengers from the Mazda, including a one-year-old baby, were taken to both John Radcliffe Hospital in Oxford and Southmead Hospital in Bristol.
The baby and a 29-year-old woman remain in a serious condition in hospital. A 24-year-old man received treatment and has since been discharged.
Those travelling in the Mazda all lived in Bedfordshire.
The lorry driver sustained minor injuries.
Road closures were put in place while a collision investigation took place but have since been lifted.
Officers would like to hear from anyone who has not already been spoken to and may have witnessed what happened. They are also appealing for anyone with dashcam footage of the collision.
Information can be submitted to police online by completing the following form and quoting incident number 98 of 17 January: https://www.gloucestershire.police.uk/tua/tell-us-about/cor/tell-us-about-existing-case-report/
Alternatively you can call police on 101, phone Crimestoppers anonymously on 0800 555 111 or visit their website: https://crimestoppers-uk.org.
Officers wish to extend their thanks to members of the public who provided assistance at the scene, as well as to colleagues from the fire and ambulance services.
The families of those involved are being supported by specially trained officers.